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陈良怡

陈良怡

研究方向：

我们发展活细胞超分辨率成像技术，并应用这些技术来研究生物医学重要问题。目前一方面的工作主要集中在引入物理光学中新成像原理、数学和信息学科中的图像重建新方法等，致力于发展可以在活细胞中实现两种以上模态光学信号探测的三维超分辨率成像的通用工具，实现同一活细胞样本上长时间、超分辨率、三维成像特定生物分子（荧光）和主要细胞器（无标记）。建立基于深度学习等手段Petabyte级的图像数据的高速处理以及分割手段，自动化、定量化描述活细胞内不同蛋白等分子以及细胞器的形状、位置以及相互作用等参数，找到新的细胞器并定义它们生化特性，最终目标是建立单细胞细胞器互作组学以及活细胞超分辨率病理学的概念，利用成像来揭示细胞内的异质性动态变化以及如代谢类疾病的发生发展机制。另一方面，我们也应用发展的高时空分辨率生物医学成像的可视化手段，系统研究血糖调控紊乱激素分泌在活体组织、细胞水平以及分子代谢水平的关系。

代表性论文：

1. Zhao W, Zhao S, Li L, Huang X, Xing S, Zhang Y, Qiu G, Han Z, Shang Y, Sun D, Shan C, Wu R, Gu L, Zhang S, Chen R, Xiao J, Mo Y, Wang J, Ji W, Chen X, Ding B, Liu Y, Mao H, Song B, Tan J, Liu J, Li H*, Chen L*. Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy, Nat Biotech. article in press.

2. Zong W, Wu R*, Chen S, Wu J, Wang H, Zhao Z, Chen G, Tu R, Wu D, Hu Y, Xu Y, Wang Y, Duan Z, Wu H, Zhang Y, Zhang Y, Wang A*, Chen L*, Cheng H. Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging, Nat Methods. 2021 Jan;18(1):46-49.

3. Zheng X, Duan R, Li L, Xing S, Ji H, Yan H, Gao K, Wang J, Wang J*, Chen L*. Live-cell superresolution pathology reveals different molecular mechanisms of Pelizaeus-Merzbacher disease, Science Bulletin, 30 December 2020; 65(24): 2061-2064.

4. Zhang Y, Wang J, Xing S, Li L, Zhao S, Zhu W, Liang K, Liu L, Chen L*, Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging, Sci China Life Sci. 2020 Oct;63(10):1543-1551.

5. Dong D, Huang X, Li L, Mao H, Mo Y, Zhang G, Zhang Z, Shen J, Liu W, Wu Z, Liu G, Liu Y, Yang H, Gong Q, Shi K*, Chen L*, Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome, Light Sci Appl. 2020 Jan 28;9:11. doi: 10.1038/s41377-020-0249-4. eCollection 2020.

6. Zhao J, Zong W, Zhao Y, Gou D, Liang S, Shen J, Wu Y, Zheng X, Wu R, Wang X, Niu F, Wang A, Zhang Y, Xiong JW, Chen L*, Liu Y*. In Vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development. Elife. 2019 Jan 29;8. pii: e41540. doi: 10.7554/eLife.41540.
Highlighted in Nature Reviews Endocrinology (2019). https://doi.org/10.1038/s41574-019-0181-y

7. Huang X, Fan J, Li L, Liu H, Wu R, Wu Y, Wei L, Mao H, Lal A, Xi P, Tang L, Zhang Y, Liu Y, Tan S*, Chen L*. Fast, long-term super-resolution imaging with Hessian structured illumination microscopy, Nat Biotech., 2018 Jun;36(5):451-459. doi: 10.1038/nbt.4115.

Highlighted in the Nature Methods (2018) https://doi.org/10.1038/s41592-018-0023-1

“2018年中国光学十大进展”; 科普视频：

https://www.youtube.com/watch?v=U9Om7DPxzd0&feature=youtu.be

8. Zong W, Wu R, Li M, Hu Y, Li Y, Li J, Rong H, Wu H, Xu Y, Lu Y, Jia H, Fan M, Zhou Z, Zhang Y*, Wang A*, Chen L*, Cheng H. Fast High-resolution Miniature Two-photon Microscopy for Brain Imaging in Freely-behaving Mice. Nat Methods. 2017 Jul;14(7):713-719.

“2017年中国科学十大进展”，“2017年中国十大医学科技新闻”和“2017年中国生命科学十大进展”; “Nature Methods: Method of the Year 2018”; 科普视频：

https://www.youtube.com/watch?v=AED0SJVAlp0

9. Yuan T, Liu L, Zhang Y, Wei L, Zhao S, Zheng X, Huang X, Boulanger J, Gueudry C, Lu J, Xie L, Du W, Zong W, Yang L, Salamero J, Liu Y*, Chen L*. Diacylglycerol Guides the Hopping of Clathrin-Coated Pits along Microtubules for Exo-Endocytosis Coupling. Dev Cell. 2015 Oct 12;35(1):120-30.

10. Zong W, Zhao J, Chen X, Lin Y, Ren H, Zhang Y, Fan M, Zhou Z, Cheng H, Sun Y*, Chen L*. Large-field high-resolution two-photon digital scanned light-sheet microscopy. Cell Res. 2015 Feb;25(2):254-7.